Artificial
Neural Network (ANN) as a Tool to Reduce Human-Animal Interaction Improves
Senegalese Sole Production
Author(s): J.M. Martínez-Vázquez, D.G. Valcarce, M.F.
Riesco, V. Sanz-Marco, M. Matsuoka, V. Robles
Source: Biomolecules, 2019
DOI: 10.3390/biom9120778
Effect of
low sperm quality on progeny: a study on zebrafish as model species.
Author(s): M.F. Riesco, D.G. Valcarce, J.M.
Martínez-Vázquez, V. Robles.
Source: Scientific Reports, 2019
DOI: 10.1038/s41598-019-47702-7
Long
Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics
Improves Sperm Quality and Progeny Survival in the Zebrafish Model
Author(s): D.G. Valcarce, M.F. Riesco, J.M.
Martínez-Vázquez, V.Robles.
Source: Biomolecules, 2019
DOI: 10.3390/biom9080338
The Use of
Antifreeze Proteins in the Cryopreservation of Gametes and Embryos.
Author(s): V. Robles, D.G. Valcarce, M.F. Riesco.
Source: Biomolecules, 2019
DOI: 10.3390/biom9050181
Non-coding
RNA regulation in reproduction: Their potential use as biomarkers.
Author(s): V. Robles, D.G. Valcarce, M.F. Riesco.
Source: Non-coding RNA Research, 2019
DOI: 10.1016/j.ncrna.2019.04.001
Diet
Supplemented with Antioxidant and Anti-Inflammatory Probiotics
Improves
Sperm Quality after Only One Spermatogenic Cycle in Zebrafish Model.
Author(s): D.G. Valcarce, M.F. Riesco, J.M.
Martínez-Vázquez, V.Robles.
Source: Nutrients, 2019
DOI: 10.3390/nu11040843
Solea senegalensis male reproductive dysfunction: new
insights into an unsolved question.
Author(s): M.F. Riesco, D.G. Valcarce, J.M.
Martínez-Vázquez, I. Martín, A. Calderón-García, V. González-Núñez, V. Robles.
Source: Reproduction, Fertility and Development, 2019
DOI: 10.1071/RD18453
Evaluation
of Intracellular Location of Reactive Oxygen Species in Solea senegalensis spermatozoa.
Author(s): D.G. Valcarce and V. Robles
Source: JoVE, 2018
DOI: doi:10.3791/55323 (2018)
Cryobanking
of aquatic species.
Author(s): S. Martínez-Páramo; A. Horvath; C. Labbé;
T. Zhang; V. Robles; P. Herráez; M. Suquet; S. Adams; A. Viveiros; T.R.
Tiersch; E. Cabrita
Source: Aquaculture 2017
DOI: 10.1016/j.aquaculture.2016.05.042
Paternal
exposure to environmental 17-alpha-ethinylestradiol concentrations modifies
testicular transcription, affecting the sperm transcript content and the
offspring performance in zebrafish.
Author(s): D.G. Valcarce; E. Vuelta; V. Robles; M.P.
Herráez
Source: Aquatic Toxicology 2017
DOI: 10.1016/j.aquatox.2017.09.025
Cryopreservation
effect on genetic function: neonatal outcomes.
Author(s): V Robles; MF Riesco; DG Valcarce
Source: Cryopreservation of mammalian gametes and
embryos: Methods and Protocols, Methods in Molecular Biology. Springer
Science+Business Media. 2017
DOI: 10.1007/078-1-4939-6828-2_19
Probiotic
administration improves sperm quality in asthenozoospermic human donors.
Author(s): D.G. Valcarce, S. Genovés, M.F. Riesco, P.
Martorell, M.P. Herráez, D. Ramón, V. Robles.
Source: Beneficial Microbes 2017
DOI: 10.3920/BM2016.0122
Paternal
contribution to development: sperm genetic damage and repair in fish.
Author(s): M.P. Herráez; J. Ausió; A. Devaux; S.
González-Rojo; C. Fernández-Díez; S. Bony; N. Saperas; V. Robles
Source: Aquaculture
DOI:10.1016/j.aquaculture.2016.03.007
Epigenetics
in fish gametes and early embryo.
Author(s): C. Labbé, Va. Robles, M.P. Herráez
Source: Aquaculture 2016
DOI:10.1016/j.aquaculture.2016.07.026
Effect of
captivity and cryopreservation on ROS production in Solea senegalensis
Author(s): D.G. Valcarce, V. Robles
Source: Reproduction 2016
DOI:10.1530/REP-16-0270
Molecular
basis of spermatogenesis and sperm quality
Author(s): V. Robles,
M.P. Herráez, C. Labbé, E. Cabrita, M.
Psenicka, D.G. Valcarce, M.F. Riesco.
Source: General and Comparative Endocrinology 2016
DOI: doi:10.1016/j.ygcen.2016.04.026
Biology of
teleost primordial germ cells (PGCs) and spermatogonia: biotechnological
applications.
Author(s): V. Robles, M.F. Riesco, M. Psenicka, T.
Saito, D.G. Valcarce, E. Cabrita, M.P. Herráez
Source: Aquaculture 2016
DOI: 10.1016/j.aquaculture.2016.03.004
Selection
of non-apoptotic sperm by magnetic activated cell sorting in Senegalese sole (Solea senegalensis)
Author(s): D.G. Valcarce, MP Herráez, O Chereguini, C
Rodríguez, V. Robles
Source: Theriogenology
DOI: 10.1016/j.theriogenology.2016.04.010
Effect of
diet supplementation with a commercial probiotic containing Pediococcus acidilactici (Lindner, 1887)
on the expression of five quality markers in zebrafish (Danio rerio (Hamilton, 1822)) testis
Author(s): Valcarce, D. G.; Pardo, M. A.; Riesco, M.
F.; et al.
Source: Journal of Applied Ichthyology Volume: 31
Pages: 18-21 Published: JUN 2015
DOI: 10.1111/jai.12731
Inhibition
of zygotic DNA repair: transcriptome analysis of the offspring in trout (Oncorhynchus mykiss)
Author(s): Fernandez-Diez, C.; Gonzalez-Rojo, S.;
Montfort, J.; et al.
Source: Reproduction Volume: 149 Issue: 1 Pages:
101-111 Published: JAN 2015
Differential
Gene Susceptibility to Sperm DNA Damage: Analysis of Developmental Key Genes in
Trout
Author(s): Gonzalez-Rojo, Silvia; Fernandez-Diez,
Cristina; Guerra, Susana M.; et al.
Source: Plos One Volume: 9 Issue: 12 Published: DEC 5
2014
DOI: 10.1371/journal.pone.0114161
Factors
enhancing fish sperm quality and emerging tools for sperm analysis
Author(s): Cabrita, E.; Martinez-Paramo, S.; Gavaia,
P. J.; et al.
Source: Aquaculture Volume: 432 Pages: 389-401
Published: AUG 20 2014
DOI: 10.1016/j.aquaculture.2014.04.034
In Vitro
Generation of Zebrafish PGC-Like Cells
Author(s): Riesco, Marta F.; Valcarce, David G.;
Alfonso, Javier; et al.
Source: Biology of Reproduction Volume: 91 Issue: 5
Published: NOV 1 2014
DOI: 10.1095/biolreprod.114.121491
Analysis of
DNA damage after human sperm cryopreservation in genes crucial for
fertilization and early embryo development
Author(s): Valcarce, D. G.; Carton-Garcia, F.;
Riesco, M. F.; et al.
Source: Andrology Volume: 1 Issue: 5 Pages: 723-730
Published: SEP 2013
DOI: 10.1111/j.2047-2927.2013.00116.x
Analysis of
transcripts in gilthead seabream sperm and zebrafish testicular cells: mRNA profile as a predictor of gamete
quality
Author(s): Guerra, S. M.; Valcarce, D. G.; Cabrita,
E.; et al.
Source: Aquaculture Volume: 406 Pages: 28-33
Published: AUG 25 2013
DOI: 10.1016/j.aquaculture.2013.04.032
Cryobiology
of cephalopod (Illex coindetii)
spermatophores
Author(s): Robles, Vanesa; Martinez-Pastor, Felipe;
Petroni, Giuliano; et al.
Source: Cryobiology Volume: 66 Issue: 3 Pages:
288-294 Published: JUN 2013
DOI: 10.1016/j.cryobiol.2013.03.005
Cryopreservation
Causes Genetic and Epigenetic Changes in Zebrafish Genital Ridges
Author(s): Riesco, Marta F.; Robles, Vanesa
Source: Plos One Volume: 8 Issue: 6 Published: JUN 21
2013
DOI: 10.1371/journal.pone.0067614
Effect of
cryopreservation on human sperm messenger RNAs crucial for fertilization and
early embryo development
Author(s): Valcarce, D. G.; Carton-Garcia, F.;
Herraez, M. P.; et al.
Source: Cryobiology Volume: 67 Issue: 1 Pages: 84-90
Published: AUG 2013
DOI: 10.1016/j.cryobiol.2013.05.007
Flow
cytometric methods for sperm assessment.
Author(s): Robles, Vanesa; Martinez-Pastor, Felipe
Source: Methods in molecular biology (Clifton, N.J.)
Volume: 927 Pages: 175-86 Published: 2013
Quantification
of lesions in nuclear and mitochondrial genes of Sparus aurata cryopreserved
sperm
Author(s): Carton-Garcia, Fernando; Riesco, Marta F.;
Cabrita, Elsa; et al.
Source: Aquaculture Volume: 402 Pages: 106-112
Published: JUL 15 2013
DOI: 10.1016/j.aquaculture.2013.03.034
Evaluation
of zebrafish (Danio rerio) PGCs
viability and DNA damage using different cryopreservation protocols
Author(s): Riesco, M. F.; Martinez-Pastor, F.;
Chereguini, O.; et al.
Source: Theriogenology Volume: 77 Issue: 1 Pages:
122-130 Published: JAN 1 2012
DOI: 10.1016/j.theriogenology.2011.07.024
Quantification
of DNA damage by q-PCR in cryopreserved zebrafish Primordial Germ Cells
Author(s): Riesco, M. F.; Robles, V.
Source: Journal of Applied Ichthyology Volume: 28
Issue: 6 Pages: 925-929 Published: DEC
2012
DOI: 10.1111/jai.12089
Refrigeration
of ram semen in presence of seminal plasma affects sperm chromatin structure
without shortening telomeres
Author(s): Mata-Campuzano, M.; Ordas, L.;
Carton-Garia, F.; et al.
Source: Reproduction in Domestic Animals Volume: 47
Pages: 97 Published: AUG 2012
Evaluation
fluorescent stains and flow cytometry for viability assessment of gilthead sea
bream (Sparus aurata) spermatozoa
Author(s): Martinez-Vazquez, R.; Martinez-Pastor, F.;
Herraez, P.; et al.
Source: Reproduction in Domestic Animals Volume: 46
Pages: 127 Published: SEP 2011
Study of
Pluripotency Markers in Zebrafish Embryos and Transient Embryonic Stem Cell
Cultures
Author(s): Robles, Vanesa; Marti, Merce; Izpisua
Belmonte, Juan Carlos
Source: Zebrafish Volume: 8 Issue: 2 Pages: 57-63
Published: JUN 2011
DOI: 10.1089/zeb.2010.0684
Cryopreservation
of fish sperm: applications and perspectives
Author(s): Cabrita, E.; Sarasquete, C.;
Martinez-Paramo, S.; et al.
Source: Journal of Applied Ichthyology Volume: 26
Issue: 5 Pages: 623-635 Published: OCT 2010
DOI: 10.1111/j.1439-0426.2010.01556.x
Regeneration
and reprogramming compared
Author(s): Christen, Bea; Robles, Vanesa; Raya,
Marina; et al.
Source: Bmc Biology Volume: 8 Published: JAN 20 2010
DOI: 10.1186/1741-7007-8-5
Cryoprotective
effects of antifreeze proteins delivered into zebrafish embryos
Author(s): Martinez-Paramo, S.; Barbosa, V.;
Perez-Cerezales, S.; et al.
Source: Cryobiology Volume: 58 Issue: 2 Pages:
128-133 Published: APR 2009
DOI: 10.1016/j.cryobiol.2008.11.013
Germplasm
Cryobanking in Zebrafish and Other Aquarium Model Species
Author(s): Robles, Vanesa; Cabrita, Elsa; Paz
Herraez, M.
Source: Zebrafish Volume: 6 Issue: 3 Pages: 281-293
Published: SEP 2009
DOI: 10.1089/zeb.2009.0592
Advances on
fish embryo cryopreservation using antifreeze proteins
Author(s): Martinez-Paramo, Sonia; Perez-Cerezales,
Serafin; Barbosa, Vera; et al.
Source: Biology of Reproduction Pages: 152 Published:
2008
Incorporation
of antifreeze proteins into zebrafish embryos by a non-invasive method
Author(s): Martinez-Paramo, S.; Perez-Cerezales, S.;
Robles, V.; et al.
Source: Cryobiology Volume: 56 Issue: 3 Pages:
216-222 Published: JUN 2008
DOI: 10.1016/j.cryobiol.2008.03.003
An
alternative method for delivering exogenous material into developing zebrafish
embryos
Author(s): Kohli, Vikram; Robles, Vanesa; Cancela, M.
Leonor; et al.
Source: Biotechnology and Bioengineering Volume: 98
Issue: 6 Pages: 1230-1241 Published: DEC 15 2007
DOI: 10.1002/bit.21564
Lipid-based
transfection as a method for gene delivery in zebrafish (Danio rerio) embryos
Author(s): Robles, Vanesa; Cancela, M. Leonor
Source: Aquaculture Research Volume: 38 Issue: 12
Pages: 1317-1322 Published: SEP 3 2007
DOI: 10.1111/j.1365-2109.2007.01806.x
Studies on
chorion hardening inhibition and dechorionization in turbot embryos
Author(s): Robles, V.; Cabrita, E.; de Paz, P.; et
al.
Source: Aquaculture Volume: 262 Issue: 2-4 Pages:
535-540 Published: FEB 28 2007
DOI: 10.1016/j.aquaculture.2006.10.034
The
antifreeze protein type I (AFP I) increases seabream (Sparus aurata) embryos tolerance to low temperatures
Author(s): Robles, V.; Barbosa, V.; Herraez, M. P.;
et al.
Source: Theriogenology Volume: 68 Issue: 2 Pages:
284-289 Published: JUL 15 2007
DOI: 10.1016/j.theriogenology.2007.05.003
Cryoprotectant
microinjection toxicity and chilling sensitivity in gilthead seabream (Sparus aurata) embryos
Author(s): Beirao, J.; Robles, V.; Herraez, M. P.; et
al.
Source: Aquaculture Volume: 261 Issue: 3 Pages:
897-903 Published: DEC 1 2006
DOI: 10.1016/j.aquaculture.2006.07.039
Microinjection
of the antifreeze protein type III (AFPIII) in turbot (Scophthalmus maximus) embryos: Toxicity and protein distribution
Author(s): Robles, V.; Cabrita, E.; Anel, L.; et al.
Source: Aquaculture Volume: 261 Issue: 4 Pages:
1299-1306 Published: DEC 11 2006
DOI: 10.1016/j.aquaculture.2006.07.047
Preliminary
studies on the cryopreservation of gilthead seabream (Sparus aurata) embryos
Author(s): Cabrita, E; Robles, V; Wallace, JC; et al.
Source: Aquaculture Volume: 251 Issue: 2-4 Pages:
245-255 Published: FEB 28 2006
DOI: 10.1016/j.aquaculture.2005.04.077
Evaluation
of DNA damage in rainbow trout (Oncorhynchus
mykiss) and gilthead sea bream (Sparus
aurata) cryopreserved sperm
Author(s): Cabrita, E; Robles, V; Rebordinos, L; et
al.
Source: Cryobiology Volume: 50 Issue: 2 Pages:
144-153 Published: APR 2005
DOI: 10.1016/j.cryobiol.2004.12.003
Evaluation
of gilthead sea bream, Sparus aurata,
sperm quality after cryopreservation in 5ml macrotubes
Author(s): Cabrita, E; Robles, V; Cunado, S; et al.
Source: Cryobiology Volume: 50 Issue: 3 Pages:
273-284 Published: JUN 2005
DOI: 10.1016/j.cryobiol.2005.02.005
Vitrification
assays with embryos from a cold tolerant sub-arctic fish species
Author(s): Robles, V; Cabrita, E; Fletcher, GL; et
al.
Source: Theriogenology Volume: 64 Issue: 7 Pages:
1633-1646 Published: OCT 15 2005
DOI: 10.1016/j.theriogenology.2005.04.014
Dimethyl
sulfoxide influx in turbot embryos exposed to a vitrification protocol
Author(s): Cabrita, E; Robles, V; Chereguini, O; et
al.
Source: Theriogenology Volume: 60 Issue: 3 Pages:
463-473 Published: AUG 2003
DOI: 10.1016/S0093-691X(03)00033-5
Effect of
different cryoprotectants and vitrificant solutions on the hatching rate of
turbot embryos (Scophthalmus maximus)
Author(s): Cabrita, E; Robles, V; Chereguini, O; et
al.
Source: Cryobiology Volume: 47 Issue: 3 Pages:
204-213 Published: DEC 2003
DOI: 10.1016/j.cryobiol.2003.10.001
Sperm
cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): parameters that affect its ability for
freezing
Author(s): Robles, V; Cabrita, E; Cunado, S; et al.
Source: Aquaculture Volume: 224 Issue: 1-4 Pages:
203-212 Published: JUN 30 2003
DOI: 10.1016/S0044-8486(03)00221-7
Vitrification
of turbot embryos: preliminary assays
Author(s): Robles, V; Cabrita, E; Real, M; et al.
Source: Cryobiology Volume: 47 Issue: 1 Pages: 30-39
Published: AUG 2003
DOI: 10.1016/S0011-2240(03)00066-X
Cryopreservation
of rainbow trout sperm in large volume straws: application to large scale
fertilization
Author(s): Cabrita, E; Robles, V; Alvarez, R; et al.
Source: Aquaculture Volume: 201 Issue: 3-4 Pages:
301-314 Published: OCT 1 2001
DOI: 10.1016/S0044-8486(01)00636-6